家蚕核型多角体病毒山东株p35基因的克隆及序列分析

根据家蚕核型多角体病毒T3株p35基因非编码区序列设计一对特异性引物，应用PCR技术从家蚕核型多角体病毒山东株中扩增得到一条1 080 bp的片段。测序结果表明，该片段含有p35基因，开放阅读框长900 bp，编码299个氨基酸，分子量为34.91kDa，pI=6.4。序列分析表明，BmNPV山东株的p35基因与T3株存在差异。Abstract:According to the 5′ and 3′ untranslated region sequences of p35 gene from Bombyx mori nuclear polyhedrosis virus isolate T3,a pair of primers was designed employing computer analysis.With the primers,polymerase chain reaction (PCR) was performed and a 1 080 bp fragment was obtained from BmNPV strain Shandong.Sequencing result showed that it was p35 gene (GenBank accession number：AY157746),and it included an open reading frame of 900 bp,encoding 299 amino acids with Mr=34.91kDa and pI=6.40.BLAST analysis indicated that there were seven bases and three amino acids difference between p35 from BmNPV strain Shandong and that from BmNPV isolate T3.

Cloning and Sequencing of p35 Gene from Bombyx mori Nuclear Polyhedrosis Virus Strain Shandong

According to the 5' and 3' untranslated region sequences of p35 gene from Bombyx mori nuclear polyhedrosis virus isolate T3, a pair of primers was designed employing computer analysis. With the primers,polymerase chain reaction (PCR) was performed and a 1080 bp fragment was obtained from BmNPV strain Shandong.Sequencing result showed that it was p35 gene (GenBank accession number: AY157746), and it included an open reading frame of 900 bp, encoding 299 amino acids with Mr=34.91 kDa and pI=6.40.BLAST analysis indicated that there were seven bases and three amino acids difference between p35 from BmNPV strain Shandong and that from BmNPV isolate T3.