| 中国西南地区特有水生植物海菜花微卫星引物开发 |
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| 引用本文： | 卢然然,杨振艳,陶程程,陈绍田,纪运恒.中国西南地区特有水生植物海菜花微卫星引物开发[J].广西植物,2014,0(1):34-37. |
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| 作者姓名： | 卢然然 杨振艳 陶程程 陈绍田 纪运恒 |
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| 作者单位： | 中国科学院昆明植物研究所生物多样性与生物地理学重点实验室;中国科学院大学; |
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| 基金项目： | 国家高技术研究发展计划项目(2010AA06Z301);国家自然科学基金(31070297,31070198) |
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| 摘 要： | 水鳖科(Hydrocharitaceae)海菜花(Ottelia acuminata)是中国西南地区特有的水生单子叶植物。基于AFLP技术的磁珠富集快速分离技术(Fast Isolation by AFLP of Sequences Containing Repeats,FIASCO),共筛选出9对多态性引物并对3个居群45个个体进行分析。结果表明:三个居群的等位基因数目为1~3个,观测杂合度从0.000~0.933,期望杂合度从0.000~0.605。这些筛选出的微卫星引物将用于海菜花后续的谱系地理学和生态遗传学研究。
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| 关 键 词： | 水鳖科 海菜花 多态 微卫星 |
Microsatellites primer development for Ottelia acuminata
(Hydrocharitaceae), a submerged macrophyte
endemic to southwestern China |
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| LU Ran-Ran,YANG Zhen-Yan,TAO Cheng-Cheng,CHEN Shao-Tian,JI Yun-Heng.Microsatellites primer development for Ottelia acuminata
(Hydrocharitaceae), a submerged macrophyte
endemic to southwestern China[J].Guihaia,2014,0(1):34-37. |
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| Authors: | LU Ran-Ran YANG Zhen-Yan TAO Cheng-Cheng CHEN Shao-Tian JI Yun-Heng |
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| Institution: | 1. Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences,
Kunming 650201, China; 2. University of Chinese Academy of Sciences, Beijing 100049, China |
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| Abstract: | Ottelia acuminata(Gagnepain)Dandy(Hydrocharitaceae),is a submerged monocot endemic to southwestern China. Using the fast isolation by AFLP of sequences containing repeats(FIASCO)protocol,nine polymorphic microsatellite loci were identified by the genotyping of forty-five individuals from three natural populations. The number of alleles(NA)per locus within populations varied from one to three. The observed and expected heterozygosities ranged from 0.000 to 0.933 and 0.000 to 0.605,respectively. These microsatellite primers can be used in future studies on the phylogeography and ecological genetics of O. acuminata. |
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| Keywords: | Hydrocharitaceae Ottelia acuminata polymorphic microsatellite |
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