Migration/sedimentation sperm selection method used in bovine in vitro fertilization: Comparison with washing/centrifugation |
| |
| Affiliation: | 1. G.EN.E.R.A. Centre for Reproductive Medicine, Clinica Valle Giulia, Via G. De Notaris 2b, 00197 Rome, Italy;2. Department of Public Health and Infectious Diseases, La Sapienza – University of Rome, Piazzale Aldo Moro 5, 00185, Rome, Italy;3. Central Queensland University, Bruce Highway, Rockhampton, 4702 Qld Australia;4. BGI Shenzhen, Beishan Industrial Zone, Yantian District Shenzhen, 518083 China;1. CARE Fertility Group, John Webster House, 6 Lawrence Drive, Nottingham Business Park, Nottingham, NG8 6PZ, UK;2. CARE Manchester, 108–112 Daisy Bank Road, Victoria Park, Manchester M14 5QH, UK;3. CARE Sheffield, 24–26 Glen Road, Sheffield S7 1RA, UK;4. CARE Northampton, 67 The Avenue, Cliftonville, Northampton NN1 5BT, UK;5. CARE Nottingham, John Webster House, 6 Lawrence Drive, Nottingham Business Park, Nottingham, NG8 6PZ, UK;6. CARE London, Park Lorne, 111 Park Rd, London NW8 7JL, UK;7. CARE Dublin, Beacon CARE Fertility, Beacon Court, Sandyford, Dublin 18, Ireland;1. Departamento de Producción Animal;2. Departamento de Medicina Veterinaria, División de Ciencias Veterinarias, CUCBA, Universidad de Guadalajara, Camino Ing. Ramón Padilla Sánchez 2100, C.P. 45220, Predio Las Agujas, Zapopan, Jalisco, Mexico;3. Laboratorio de Genética Molecular, Universidad Autónoma de Nayarit, Posgrado CBAP, Ciudad de la Cultura, Amado Nervo s/n, C.P. 63155 Tepic, Nayarit, Mexico |
| |
| Abstract: | Sperm selection methods are usually considered for in vitro fertilization (IVF) programs. To achieve a population of viable, motile and morphologically normal spermatozoa, seminal plasma and contaminants must be removed. In this study 2 sperm selection methods were compared with regard to conventional parameters: 1) repeated washing/centrifugation, and 2) migration/sedimentation. Their effect on the fertilization of oocytes aspired from ovaries of slaughtered cows was determined. Frozen-thawed semen was used for all assays (n = 8). The sperm concentration was adjusted to 1.0 × 106 cells/ml for in vitro insemination. Selected oocytecumulus complexes were matured in vitro for 24 h and were co-incubated with spermatozoa prepared by the 2 different methods. Presumptive zygotes were co-cultered in TCM-199. The percentage of motile, viable and morphologically normal spermatozoa was markedly higher (p < 0.05) with the migration-sedimentation method. Triple stain showed that the integrity of the acrosome was significantly better maintained after migration/sedimentation (68.3%) than after washing/centrifugation (53.2%; p < 0.05). In accordance with these results, a high fertilisation rate was achieved after application of the migration/sedimentation method (92.5 vs 69.1%;p < 0.05). It is concluded, that this method is very promising for obtaining spermatozoa with optimal fertilization capacity. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
