Selective modulation of membrane sphingomyelin fatty acid turnover by Nigericin. A study in the rat reticulocyte |
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| Affiliation: | 1. Department of Analytical Chemistry, Stockholm University, S-106 91 Stockholm, Sweden;2. Science for Life Laboratory, Division of Translational Medicine and Chemical Biology, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S-171 21 Stockholm, Sweden;1. Department of Pharmaceutical and Medicinal Chemistry, Faculty of Pharmacy, University of Port Harcourt, Rivers State, Nigeria;2. Department of Pharmaceutical and Medicinal Chemistry, University of Nigeria Nsukka, Enugu State, Nigeria;1. Obstetrics, Nutrition and Endocrinology Group, Department of Obstetrics and Gynaecology, University of Melbourne, Victoria, Australia;2. Mercy Perinatal Research Centre, Mercy Hospital for Women, Heidelberg, Victoria, Australia;3. Translational Obstetrics Group, Department of Obstetrics and Gynaecology, University of Melbourne, Victoria, Australia |
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| Abstract: | Exposure of rat reticulocytes to Nigericin produced a selective modulation of fatty acid incorporation into sphingomyelin (SM) of the cell membrane, via changes in SM acylation kinetics. At physiological fatty acid concentration, Nigericin accelerated 8-fold SM acylation by decreasing the apparent Km for oleate from 14.7 μM to 2.0 μM. The response was diminished in high K+-containing media, suggesting an effect of Nigericin as K+ transporter. This constitutes a novel piece of evidence for the important role of ions in SM metabolism. |
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