Effect of testosterone and dibutyryl c-AMP on the meiotic competence in pig oocytes of various size categories |
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| Institution: | 1. Research Institute of Animal Production Department of Reproductive Biology 104 00 Praha 10 — Uh?íněves, Czech Republic;2. University of Agriculture Department of Veterinary Sciences 165 21 Praha 6 — Suchdol, Czech Republic;1. School of Psychology and Life Sciences, Canterbury Christ Church University, Canterbury, CT1 1QU, UK;2. Topigs Norsvin, Helvoirt, The Netherlands;1. School of Medical Sciences, Faculty of Medicine and Health, University of Sydney, New South Wales, Australia;2. Priority Research Centre for Reproductive Science, School of Environmental and Life Sciences, Faculty of Science, University of Newcastle, Callaghan, New South Wales, Australia;3. MP Fertility, Central Coast, New South Wales, Australia;1. State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing, 100193, China;2. Beijing Advanced Innovation Center for Food Nutrition and Human Health, China Agricultural University, Beijing, 100193, China;3. Laboratory Animal Center of the Academy of Military Medical Sciences, Beijing, 100071, China;4. Department of Food and Bioengineering, Beijing Vocational College of Agriculture, Beijing, 102442, China;1. MRC Laboratory for Molecular Cell Biology, University College London, London WC1E 6BT, UK;2. Cancer Research UK London Research Institute, Clare Hall Laboratories, South Mimms, Hertfordshire, EN6 3LD, UK;3. Department of Cellular Logistics, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany;4. Institute for Research in Immunology and Cancer, Université de Montréal, Montreal QC, H3T 1J4, Canada;5. London Centre for Nanotechnology, University College London, London WC1H 0AH, UK;6. Department of Cell and Developmental Biology, University College London, London WC1E 6BT, UK;7. Department of Pathology and Cell Biology, Faculty of Medicine, Université de Montréal, Montreal, QC, H3T 1J4, Canada |
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| Abstract: | Spontaneous meiosis resumption was investigated in small pig oocytes of various size categories. Two parameters were studied: 1) the possibility for inhibiting spontaneous meiosis resumption in small oocytes of various size categories and 2) the level of meiotic competence of small oocytes in which meiosis resumption had been temporarily blocked. It was observed that 1 mM dibutyryl cyclic adenosine monophosphate (dbcAMP) combined with 0.5 μM testosterone were able to prevent spontaneous maturation in pig oocytes of various size categories cultured in vitro for up to 10 d. Moreover, a culture of these oocytes with dbcAMP and testosterone has a beneficial effect on the integrity of the oocyte-granulosa cell complex. When oocytes were allowed to mature after long-term inhibition of maturation, the percentage of oocytes able to resume maturation was significantly increased. However, the portion of oocytes which completed maturation, reaching the metaphase II (M II) stage, did not increase. It was found that dbcAMP, in combination with testosterone, also efficiently inhibited spontaneous maturation in fully grown pig oocytes. When the inhibitory effect of dbcAMP and testosterone was reversed in fully grown oocytes, maturation was significantly accelerated. |
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