Reversed-phase high-performance liquid chromatographic screening method for serum steroids using retention index and diode-array detection |
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| Authors: | Pirjo Kuronen Pirkko Volin Tarja Laitalainen |
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| Institution: | Department of Chemistry, Laboratory of Organic Chemistry, P.O. Box 55, University of Helsinki FIN-00014, Finland |
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| Abstract: | A multisteroid screening method has been developed based on the use of 1-4-(2,3-dihydroxypropoxy)phenyl]-1-alkanones as retention index standards and UV absorbance spectra recorded on-line with a diode-array detector using reversed-phase high-performance liquid chromatographic gradient elution with acetonitrile and water. The effect of chromatographic conditions on retention indices of steroids were studied. The method was tentatively applied to profiling of steroids in serum samples. |
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| Keywords: | Steroids |
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