Monocarboxylate transporter genes in the mammary gland of lactating cows |
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Authors: | Doaa Kirat Seiyu Kato |
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Institution: | (1) Department of Veterinary Physiology, School of Veterinary Medicine, Rakuno Gakuen University, 582-1 Bunkyodai-Midorimachi, Ebetsu Hokkaido, 069-8501, Japan;(2) Department of Physiology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, 44519, Egypt |
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Abstract: | This study is the first to examine the expression of the 14 monocarboxylate transporter genes (MCT1–MCT14) in the mammary
gland of mammals. RT-PCR, Western blot, immunohistochemistry, and immunofluorescence confocal laser microscopy were applied
in a comprehensive approach to assess the expression and cellular localization of MCTs in the mammary gland of lactating cattle.
RT-PCR revealed the existence of nine MCT isoforms, namely MCT1, MCT2, MCT3, MCT4, MCT5, MCT8, MCT10, MCT13, and MCT14 in
cow mammary gland. The amplified cDNA segments were confirmed by sequence analysis and deposited in the GenBank. Using the
commercially available antibodies against MCT1–MCT8, Western blotting verified the protein expression of MCT1, MCT2, MCT3,
MCT4, MCT5, and MCT8 in the cow mammary gland. The precise cellular localization of the identified MCT proteins showed that
both MCT1 and MCT2 were basolaterally localized on the cow mammary alveolar epithelial cells. In contrast, MCT4 protein signal
was expressed on the apical membrane of these alveolar epithelia. MCT8, however, was predominantly localized on the basolateral
membranes of the lactocytes, along with its weak labeling on the apical membrane of the same cells. No immunoreactive staining
for MCT3 and MCT5 proteins could be detected histochemically in lactating bovine mammary tissue. Additionally, we proved the
colocalization of CD147 with both MCT1 and MCT4 on the boundaries of the cow mammary alveolar epithelia. The existence and
localization pattern of MCT genes in the mammary gland of lactating cows suggest their possible involvement in the transport
of essential elements required for milk synthesis and secretion. |
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