Determination of phosphatidylethanol in blood from alcoholic males using high-performance liquid chromatography and evaporative light scattering or electrospray mass spectrometric detection |
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| Authors: | Torsten Gunnarsson,Anders Karlsson,Per Hansson,Gö ran Johnson,Christer Alling,Gö ran Odham |
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| Affiliation: | aCamurus AB, Ideon, Gamma 1, Sölvegatan 41, SE-223 70 Lund, Sweden;bNycomed Innovation AB, Ideon-Malmö, SE-205 12 Malmö, Sweden;cDepartment of Medical Neurochemistry, Institute of Laboratory Medicine, Lund University, SE-221 85 Lund, Sweden;dDepartment of Alcohol and Drug Diseases, Malmö University Hospital, SE-205 02 Malmö, Sweden;eLaboratory for Analytical Environmental Chemistry, Stockholm University, SE-106 92 Stockholm, Sweden |
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| Abstract: | The `pathologic' phospholipid, phosphatidylethanol (PEth), formed only in the presence of ethanol, was determined in extracts of human blood using high-performance liquid chromatography with evaporative light scattering detection (ELSD) or electrospray (ES) mass spectrometry. Separation was performed using a diol column and a normal-phase binary gradient system. Decreasing concentrations of PEth (15 to 1 nmol/ml blood) could be detected by ELSD in three male alcoholics, up to 3 weeks after the beginning of an alcohol-free period. Using ES, levels down to 100 pmol/ml blood was detected. The molecular species of PEth were similar to those of phosphatidylcholine found in the same blood sample. The method provides a rapid quantitative and qualitative determination of PEth in blood. The limits of detection were 200 pmol (≈125 ng) using ELSD and 140 fmol (≈100 pg) using ES, total amounts injected. |
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| Keywords: | Phosphatidylethanol Phospholipids |
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