Comparison of detection methods for liquid chromatographic determination of 3-nitro-l-tyrosine |
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| Authors: | Huwei Liu Tiehua Huang Candice B Kissinger Peter T Kissinger |
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| Affiliation: | aDepartment of Chemistry, Purdue University, West Lafayette, IN 47907, USA;bBioanalytical Systems, Inc., 2701 Kent Ave., West Lafayette, IN 47906, USA;cDepartment of Chemistry, Peking University, Beijing 100871, China |
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| Abstract: | A liquid chromatographic method has been developed for the determination of 3-nitro-l-tyrosine. Different detection methods, including UV, oxidative and redox electrochemistry, and postcolumn photolysis followed by electrochemical detection, have been optimized and compared in terms of analysis time, detection limit and dynamic range. It was demonstrated that liquid chromatography with postcolumn photolysis followed by electrochemical detection is the most effective method, with an analysis time of 5 min, detection limit of 0.01 pmol, and a linear dynamic range from 2 nM to 100 μM. |
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| Keywords: | 3-Nitro- font-variant: small-caps" >l-tyrosine |
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