Liquid chromatographic determination of myocardial interstitial epinephrine |
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| Authors: | Toru Kawada Toji Yamazaki Tsuyoshi Akiyama Takayuki Sato Toshiaki Shishido Masaru Sugimachi Masashi Inagaki Joe Alexander Jr. Kenji Sunagawa |
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| Affiliation: | aDepartment of Cardiovascular Dynamics, National Cardiovascular Center Research Institute, 5-7-1 Fujishirodai, Suita, Osaka 565, Japan;bDepartment of Cardiac Physiology, National Cardiovascular Center Research Institute, 5-7-1 Fujishirodai, Suita, Osaka 565, Japan;cDepartment of Biomedical Engineering, Vanderbilt University, Nashville, TN 37235, USA |
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| Abstract: | This study describes a high-performance liquid chromatographic method with electrochemical detection (HPLC–ED) for monitoring of epinephrine (Epi) in the myocardial interstitial space. The in vitro detection limit for Epi was 200 fg in a 50-μl injection. Using a cardiac dialysis technique, 60-μl dialysates were sampled from the myocardial interstitial space (6-min fractions). After an alumina procedure, the dialysate Epi concentration was measured using the HPLC–ED system. Although the basal Epi concentration was undetectable, local administration of desipramine increased Epi concentration of the dialysate to 38.1±18.5 pg/ml. This system affords a new possibility for estimating myocardial interstitial Epi level. |
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| Keywords: | Epinephrine |
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