An improved optical technique for monitoring plant cell concentration |
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Authors: | E. James J. M. Lee |
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Affiliation: | (1) Department of Chemical Engineering, Washington State University, Pullman, WA 99164–2710, USA e-mail: jmlee@mail.wsu.edu Fax: +1-509-335-4806, US |
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Abstract: | An improved optical method was developed to determine cell weight concentrations using a micro-plate reader. Light absorbance was measured by a vertical light beam, which can minimize the cell settling effect found in traditional optical measurements with a horizontal light beam. The use of well plates not only requires very small sample sizes, but also handles a large number of samples at the same time. Absorbance measurements were linearly related to cell weight over the full range of batch culture growth. Received: 4 Jannuary 1999 / Revision received: 23 March 1999 / Accepted: 14 April 1999 |
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Keywords: | Dry cell weight Turbidity Plant cells Optical method |
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