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An improved optical technique for monitoring plant cell concentration
Authors:E. James  J. M. Lee
Affiliation:(1) Department of Chemical Engineering, Washington State University, Pullman, WA 99164–2710, USA e-mail: jmlee@mail.wsu.edu Fax: +1-509-335-4806, US
Abstract: An improved optical method was developed to determine cell weight concentrations using a micro-plate reader. Light absorbance was measured by a vertical light beam, which can minimize the cell settling effect found in traditional optical measurements with a horizontal light beam. The use of well plates not only requires very small sample sizes, but also handles a large number of samples at the same time. Absorbance measurements were linearly related to cell weight over the full range of batch culture growth. Received: 4 Jannuary 1999 / Revision received: 23 March 1999 / Accepted: 14 April 1999
Keywords:  Dry cell weight  Turbidity  Plant cells  Optical method
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