LC–MS/APCI identification of glucoside esters and diesters of astaxanthin from the snow alga Chlamydomonas nivalis including their optical stereoisomers |
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| Institution: | 1. Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, 142 20 Prague, Czech Republic;2. Charles University in Prague, Faculty of Science, Department of Ecology, Viničná 7, 128 44 Prague 2, Czech Republic;3. Institute of Botany, Academy of Sciences of the Czech Republic, Dukelská 135, 379 82 Třeboň, Czech Republic;4. Department of Biotechnology, Institute of Chemical Technology Prague, Technická 5, 166 28 Prague, Czech Republic;1. School of Bio Sciences and Technology, VIT University, Vellore 632014, Tamil Nadu, India;2. Department of Environmental and Biological Chemistry, Chungbuk National University, Chungbuk, Cheongju, Republic of Korea |
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| Abstract: | HPLC methods (LC–MS/APCI and chiral HPLC) were used for the identification of astaxanthin derivatives from the red snow alga Chlamydomonas nivalis collected in Austrian Alps, Slovak High Tatra Mountains and Bulgarian Pirin. We observed a striking difference in the composition of astaxanthin optical isomers in C. nivalis collected in geographically distinct regions. Furthermore, algae from the Pirin Mountains differed in the dominance of astaxanthin diglucoside diesters, suggesting an alternative strategy to enhance cell viability at low temperatures. |
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