| Institution: | 1. Department of Biology, University of Missouri, St. Louis, MO 63121, United States;2. Donald Danforth Plant Science Center, St. Louis, MO 63132, United States;3. Kansas Lipidomics Research Center, Division of Biology, Kansas State University, Manhattan, KS 66506, United States;1. Department of Animal Science, National Chung Hsing University, Taichung, Taiwan, Republic of China;2. Division of Animal Science, School of Agriculture and Natural Resources, University of Phayao, Phayao, Thailand;3. Department of Animal Science, Faculty of Agriculture, Ubon Ratchathani University, Ubon Ratchathani, Thailand;4. Division of Biotechnology, Agriculture Technology Research Institute, Hsinchu City, Taiwan, Republic of China;5. Department of Medical Research, Taichung Veterans General Hospital, Taichung, Taiwan, Republic of China;6. Departement d''Elevage, Institut du Developpement Rural, Universite Polytechnique de Bobo, Bobo Dioulasso, Burkina Faso;7. Department of Animal Science and Biotechnology, Tunghai University, Taichung, Taiwan, Republic of China;8. Department of Biomedical Informatics and Medical Engineering, Asia University, Taichung, Taiwan, Republic of China;9. Core Laboratory for Stem Cell Research, Medical Research Department, China Medical University Hospital, Taichung, Taiwan, Republic of China;1. University Hospital Quiron Dexeus, Hip Unit - Department of Orthopaedic Surgery, Barcelona, Spain;2. Hospital de la Santa Creu i Sant Pau, Universitat Autònoma de Barcelona, Barcelona, Spain;3. Hospital General de Catalunya, Barcelona, Spain;1. Department of Biology, Indiana University-Purdue University Fort Wayne, 2101 East Coliseum Blvd., Fort Wayne, IN 46805, USA;2. Department of Plant Pathology and Ecology, The Connecticut Agricultural Experiment Station, 123 Huntington St, New Haven, CT 06511, USA;1. Cell & Molecular Sciences, The James Hutton Institute, Invergowrie, Dundee, DD2 5DA, United Kingdom;2. Institute of Mechanical, Process and Energy Engineering, School of Engineering and Physical Sciences, Heriot-Watt University, Edinburgh, EH14 4AS, United Kingdom;3. Environmental & Biochemical Sciences, The James Hutton Institute, Invergowrie, Dundee, DD2 5DA, United Kingdom |
| Abstract: | The availability of nitrogen (N) to plants has a profound impact on carbohydrate and protein metabolism, but little is known about its effect on membrane lipid species. This study examines the changes in galactolipid and phospholipid species in soybean as affected by the availability of N, either supplied to soil or obtained through Bradyrhizobium japonicum nodulation. When N was limited in soil, the content of galactolipids, monogalactosyldiacylglycerol (MGDG) and digalactosyldiacyglycerol (DGDG), decreased drastically in leaves, while a smaller decrease of DGDG was observed in roots. In both leaves and roots, the overall content of different phospholipid classes was largely unchanged by N limitation, although some individual phospholipid molecular species did display significant changes. Nodulation with Bradyrhizobium of soybean grown in N-deficient soil resulted in a large increase in levels of plastidic lipid classes, MGDG, DGDG, and phosphatidylglycerol, along with smaller increases in non-plastidic phospholipids in leaves. Nodulation also led to higher levels of phospholipids in roots without changes in root levels of MGDG and DGDG. Overall, N availability alters lipid content more in leaves than roots and more in galactolipids than phospholipids. Increased N availability leads to increased galactolipid accumulation in leaves, regardless of whether N is supplied from the soil or symbiotic fixation. |
