Restriction fragment length polymorphism of the caprine ß-globin gene cluster associated with the Hb ß-globin variants in Norwegian dairy goats |
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Authors: | O BERGERSEN M BRAEND |
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Institution: | Norwegian College of Veterinary Medicine, Department of Animal Genetics, National Veterinary Institute, Oslo. |
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Abstract: | Restriction fragment length polymorphism (RFLP) analysis was used to characterize the beta-globin cluster in Norwegian dairy goats. In 122 individuals, different RFLP patterns were detected after BglII and PstI digestion and hybridization with a caprine beta-globin probe. The location of the polymorphic BglII and PstI sites were determined. The different RFLPs were in linkage disequilibrium with each other and with the beta-globin locus as studied at the protein level. A preferential association between certain RFLP haplotypes and beta-globin variants was observed. Polymorphic DNA fragments in the epsilon-globin genes were detected after MspI digestion and hybridization with a caprine epsilon-probe, and eight different band patterns were observed. Correlation between different MspI haplotypes and the beta-globin variants was determined. |
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Keywords: | Keywords: goat haemoglobin RFLP ß-globin genes linkage disequilibrium |
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