NMDA-induced stimulation of glycolysis in developing hippocampal cell cultures |
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| Authors: | Jiraporn Luengviriya Carina Helmeke Katharina Braun Marcus J B Hauser Thomas Mair |
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| Institution: | (1) Insitute of Experimental Physics, Department of Biophysics, Otto-von-Guericke University, 39106 Magdeburg, Germany;(2) Department of Industrial Physics and Medical Instrumentation, King Mongkut’s University of Technology North Bangkok, Bang Sue, 10800 Bangkok, Thailand;(3) Department of Zoology/Developmental Neurobiology, Otto-von-Guericke University, 39120 Magdeburg, Germany |
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| Abstract: | Developmental changes in energy metabolism of primary hippocampal cell cultures from newborn rats were investigated during
the first 3 weeks. These changes were measured by intensity of and number of cells exhibiting NAD(P)H fluorescence in response
to NMDA-induced activation of neuronal activity. We observed gradual changes of stimulation-evoked NAD(P)H signaling over
the first 3 weeks, such that at day 7 and 16, this stimulation is minimal, while at 5 and 12 days, it is maximal. These results
describe a biphasic pattern that was similar to earlier findings from experiments investigating developmental changes in population
spike amplitudes or glutamate release in young rats. Inhibition of mitochondrial respiration by KCN revealed that the NMDA-evoked
stimulation of energy metabolism is mainly due to increased glycolytic activity. |
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| Keywords: | NAD(P)H fluorescence Cyanide Neurometabolic coupling Biphasic response |
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