Identification of cyclic AMP-CRP binding sites in the intercistronic regulatory uxaCA-exuT region of Escherichia coli

Abstract In Escherichia coli K-12 the two adjacent operons exuT and uxaCA are divergently transcribed and each possesses its own control region. We show that in vivo the expression of these two operons seems to be sensitive to catabolite repression and to be cAMP-dependent. The nucleotide sequence of 318 nucleotides, including the entire control region of uxaCA and the exuT operator, was determined and two cAMP-CRP binding sites were identified.