Ultrastructural analysis of the sperm cells of mature pollen of maize,Zea mays |
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Authors: | C A McConchie T Hough R B Knox |
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Institution: | (1) Plant Cell Biology Research Centre, School of Botany, University of Melbourne, 3052 Parkville, Vic., Australia;(2) Present address: Zellenlehre, Universität Heidelberg, Im Neuenheimer Feld 230, Germany |
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Abstract: | Summary Ultrastructural analysis of the mature viable unhydrated pollen of maize,Zea mays from dehiscent anthers shows that the sperm cells are physically distant, each bounded by an envelope comprising their own plasma membrane and the inner plasma membrane of the vegetative cell. The chondriome is unusual in containing one or more filamentous complexes, up to 12 m in length appressed to the side of the sperm nucleus. The extensions at each end of the elongate sperm cells contain longitudinally-oriented arrays of endoplasmic lamellae. In a three-dimensional reconstruction of serial thin sections, there is a long J-shaped sperm, c. 35 × 5 m and up to 1 m in thickness, sited within pointed evaginations of the vegetative nucleus and a second shorter sperm c. 20 × 5 m and up to 3.5 m in thickness.Abbreviations PA-TCH-SP
periodic acid-thiocarbohydrazide-silver proteinate
- DAPI
4,6-diamino-2-phenylindole
- SC
sperm cell
- Sn
sperm nucleus
- Ua-Pb
Uranyl acetate-lead citrate staining
- ER
endoplasmic reticulum |
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Keywords: | Sperm cells Pollen Maize Chondriome Three-dimensional reconstruction |
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