绿色荧光蛋白基因在转基因小鼠体内的表达

建立绿色荧光蛋白（GFP）转基因小鼠，继而传代建系。采用显微注射法，将GFP基因注入FVB／NJ小鼠受精卵原核内，获得子代鼠。分娩后3周剪取仔鼠尾，提取基因组DNA，应用PCR、Southern印迹技术进行整合检测。结共用雌性小鼠200只，注射受精卵1586枚，移植卵数386枚，受体鼠32只，怀孕鼠4只，子代鼠18只，有4只为阳性：取2只首建鼠的胚胎，在荧光显微镜下观察GFP表达明显，表明初步获得了转绿色荧光蛋白基因小鼠，

Construction of Transgenic Mice Carrying Enhanced Green Fluorescent Protein Gene by microinjection

It was attempted to develop green fluorescent protein(GFP) transgenic mouse and produce its lineages. GFP gene was introduced into the zygote of FVB/NJ mice by microinjection. The tail of GFP mice obtained from the founders of three mouths mice and picked-up the genome of mice, genomic DNA was extracted from the offspring of the founder mice for PCR and Southern blotting analysis. GFP gene was injected into pronucleus of 1 586 zygotes and 386 injected eggs were implanted into 32 foster mothers, 4 of which were pregnant and bore 13 off springs. Among the babies, 4 offsprings were integrated with the GFP gene and expressed it. The embryo of 2 founders mice were observed for fluorescence microscopy observation and succeed to detect GFP expression in the embryo of the founder mice. The model of transgenic GFP was established primarily.