Nucleotide sequence of the gene encoding the Corynebacterium glutamicum mannose enzyme II and analyses of the deduced protein sequence |
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Authors: | Jung-Kee Lee Moon-Hee Sung Ki-Hong Yoon Ju-Hyun Yu Tae-Kwang Oh |
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Affiliation: | Genetic Engineering Research Institute, Korea Institute of Science and Technology, PO Box 115, Yusung, Taejon 305-600, South Korea; Department of Food and Biotechnology, College of Engineering, Yonsie University, Seoul 120-749, South Korea |
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Abstract: | Abstract The complete nucleotide sequence of the gene encoding the Corynebacterium glutamicum mannose enzyme II (EIIMan) was determined. The gene consisted of 2052 base pairs encoding a protein of 683 amino acid residues; the molecular mass of the protein subunit was calculated to be 72570 Da. The N-terminal hydrophilic domain of EIIMan showed 39.7% homology with a C-terminal hydrophilic domain of Escherichia coli glucose-specific enzyme II (EIIGlc). Similar homology was shown between the C-terminal sequence of EIIMan and the E. coli glucose-specific enzyme III (EIIIGlc), or the EIII-like domain of Streptococcus mutans sucrose-specific enzyme II. Sequence comparison with other EIIs showed that EIIMan contained residues His-602 and Cys-28 which were homologous to the potential phosphorylation sites of EIIIGlc, or EIII-like domains, and hydrophilic domains (IIB) of several EIIs, respectively. |
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Keywords: | Phosphoenolpyruvate sugar phosphotransferase system Sugar transport Mannose enzyme II Corynebacterium glutamicum |
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