Nucleotide regulation of vasoactive intestinal peptide binding to bovine thyroid plasma membranes |
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Authors: | Juan R. Calvo María F. Morales Juan C. Mesa Raimundo Goberna |
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Affiliation: | (1) Department of Medical Biochemistry and Molecular Biology, School of Medicine, University of Sevilla, Avda. Sánchez Pizjuán, 4 41009 Sevilla, (Spain) |
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Abstract: | The specific binding of vasoactive intestinal peptide (VIP) to bovine thyroid plasma membranes is inhibited by guanine nucleotides. Guanosine 5-triphosphate (GTP) and the non-hydrolyzable GTP analogs guanosine 5-,-imidotriphosphate (Gpp(NH)p) and guanosine 5-O-(3-thiotriphosphate) (GTP--S) inhibited markedly the binding of VIP to its receptors. This inhibition was higher with GTP than with Gpp(NH)p and GTP--S and was due to an increase of the rate of dissociation of peptide bound to membranes. Other nucleotides did not show any effect. |
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Keywords: | vasoactive intestinal peptide binding thyroid plasma membranes nucleotide regulation |
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