Characterization of calcium/calmodulin-dependent protein kinase II activity in the nervous system of the lobster,Panulirus interruptus |
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Authors: | Dr Michelle D Withers Mary B Kennedy Eve Marder Leslie C Griffith |
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Institution: | (1) Volen Center and Biology Department, Brandeis University, 02254 Waltham, MA, USA;(2) Division of Biology, California Institute of Technology, 91125 Pasadena, CA, USA |
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Abstract: | Nervous system tissue fromPanulirus interruptus has an enzyme activity that behaves like calcium/calmodulin-dependent protein kinase II (CaM KII). This activity phosphorylates
known targets of CaM KII, such as synapsin I and autocamtide 3. It is inhibited by a CaM KII-specific autoinhibitory domain
peptide. In addition, this lobster brain activity displays calcium-independent activity after autophosphorylation, another
characteristic of CaM KII. A cDNA from the lobster nervous system was amplified using polymerase chain reaction. The fragment
was cloned and found to be structurally similar to CaM KII. Serum from rabbits immunized with a fusion protein containing
part of this sequence immunoprecipitated a CaM KII enzyme activity and a family of phosphoproteins of the appropriate size
for CaM KII subunits.
Lobster CaM KII activity is found in the brain and stomatogastric nervous system including the commissural ganglia, commissures,
stomatogastric ganglion and stomatogastric nerve. Immunoblot analysis of these same regions also identifies bands at an apparent
molecular weight characteristic of CaM KII. |
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Keywords: | stomatogastric nervous system crustaceans |
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