UV and Ethyl Methanesulfonate Effects in Hyperthermophilic Archaea and Isolation of Auxotrophic Mutants of Pyrococcus Strains |
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Authors: | Laurent Watrin Daniel Prieur |
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Institution: | (1) Marine Microbiology, Station Biologique, Centre National de la Recherche Scientifique, UPR 9042, Place G. Teissier, BP 74, 29682 Roscoff Cedex, France , FR |
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Abstract: | The lethal and mutagenic effects of ethyl methanesulfonate (EMS) and UV on nine archaeal strains belonging to each of the
two described genera of Thermococcales, Pyrococcus and Thermococcus, were investigated. To test the efficiency of the EMS and UV mutagenesis under a variety of experimental conditions, we chose
Pyrococcus abyssi strain GE5 as a model strain. We observed a strong induced mutagenicity in both cases, since the spontaneous mutation frequency
(expressed as the frequency of resistance to 5-fluoroorotic acid) increased up to 150-fold with EMS and 400-fold with UV,
after mutagen exposure. Although a heterogeneous response to the induced effects caused after EMS and UV exposures was detected
for all the other sulfothermophilic archaea tested, an efficient mutagenicity of Pyrococcus-like isolates GE27, GE23, and GE9 was observed. Optimal procedures described for UV mutagenesis yielded a number of useful
uracil auxotrophic mutant strains of Pyrococcus abyssi.
Received: 2 May 1996 / Accepted: 3 July 1996 |
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