| 东亚飞蝗谷胱甘肽S-转移酶RNA干扰效率研究 |
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| 引用本文： | 刘婷,秦国华,张建珍,马恩波. 东亚飞蝗谷胱甘肽S-转移酶RNA干扰效率研究[J]. 昆虫知识, 2011, 48(4): 820-825 |
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| 作者姓名： | 刘婷 秦国华 张建珍 马恩波 |
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| 作者单位： | 1. 山西大学应用生物学研究所,太原,030006
2. 山西大学应用生物学研究所,太原,030006;山西大学环境与资源学院,太原,030006 |
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| 基金项目： | 国家自然科学基金重大国际合作项目，国家自然科学基金，中国博士后科学基金 |
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| 摘 要： | 东亚飞蝗Locusta migratoria manilensis(Meyen)是我国主要的农业害虫之一,已发现东亚飞蝗对某些农药产生了抗性,其抗性机制可能与谷胱甘肽硫转移酶(GST)代谢解毒相关.本研究利用特异性引物合成东亚飞蝗GST 4个不同家族基因的双链RNA(dsRNA),将dsRNA注射到东亚飞蝗幼虫体内,采...
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| 关 键 词： | 谷胱甘肽硫转移酶 东亚飞蝗 RNA干扰 时间效应 |
The RNA interference efficiency of glutathione S-transferases from Locusta migratoria manilensis |
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| LIU Ting,QIN Guo-Hua,ZHANG Jian-Zhen,MA En-Bo. The RNA interference efficiency of glutathione S-transferases from Locusta migratoria manilensis[J]. Entomological Knowledge, 2011, 48(4): 820-825 |
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| Authors: | LIU Ting QIN Guo-Hua ZHANG Jian-Zhen MA En-Bo |
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| Affiliation: | LIU Ting1 QIN Guo-Hua 1,2 ZHANG Jian-Zhen1 MA En-Bo1 (1.Institute of Applied Biology,Shanxi University,Taiyuan 030006,China,2.College of Environment and Resource,China) |
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| Abstract: | The migratory locust,Locusta migratoria manilensis (Meyen) (Orthoptera: Acridoidea),is one of the most important pests in our country.Resistance of this locust to some insecticides has been detected and the resistance mechanism hypothesized to involve glutathione S-transferases (GST).In this study,double-stranded RNA of four different classes of GST was synthesized using specific gene primers and injected into the 2nd instar nymphs of L.m.manilensis.The effects of silencing time on target GSTs mRNA expressi... |
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| Keywords: | glutathione S-transferases (GSTs) Locusta migratoria manilensis RNAi time effect |
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