Light-regulated betacyanin accumulation in euhalophyte <Emphasis Type="Italic">Suaeda salsa</Emphasis> calli |
| |
Authors: | Shu-Zhen Zhao Hong-Zhao Sun Min Chen Bao-Shan Wang |
| |
Institution: | (1) Key Laboratory of Plant Stress Research, College of Life Science, Shandong Normal University, No.88, East Wenhua Road, Jinan, 250014, People’s Republic of China;(2) Department of Biological Science and Technology, Shandong Institute of Education, No.36, Lishan Road, Jinan, 250013, People’s Republic of China |
| |
Abstract: | Suaeda salsa calli cultured in darkness for 28 days were transferred to Murashige and Skoog (MS) media containing 0.5 mg l−1 Thidiazuron (TDZ) and 1.0 mg l−1 naphthaleneacetic acid (NAA) under different light conditions for 10 days to investigate effect of light on betacyanin accumulation
and its relation to activity and expression of tyrosinase and expression of dopa-4,5-dioxygenase gene (DODA). Both light quality and quantity affected betacyanin synthesis of S. salsa calli. 80 μmol m−2 s−1 of white light intensity was optimal for cell growth and betacyanin accumulation of S. salsa calli. DODA mRNA levels and tyrosinase activity were consistent with the response of betacyanin content to different light quality. However,
the protein levels of tyrosinase extracted from S. salsa calli response to different light quality were constant. These results suggest that betacyanin metabolism in S. salsa calli is regulated by different light quality through the regulation of genes such as DODA and tyrosinase activity but not via alteration of the protein amount of tyrosinase. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|