Effects of nitric oxide scavengers on thermoinhibition of seed germination in <Emphasis Type="Italic">Arabidopsis thaliana</Emphasis> |
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Authors: | K K Hossain R D Itoh G Yoshimura G Tokuda H Oku M F Cohen H Yamasaki |
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Institution: | 1.Faculty of Science,University of the Ryukyus,Nishihara, Okinawa,Japan;2.Center of Molecular Biosciences (COMB),University of the Ryukyus,Nishihara, Okinawa,Japan;3.Department of Biology,Sonoma State University,Rohnert Park California,USA |
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Abstract: | Plant seeds sometimes do not germinate at elevated temperature. The thermoinhibition mechanisms of seed germination have yet
not revealed. Here we describe a chemical approach to improve seed germination at high temperature. We compared the temperature
response of germination between wild-type Arabidopsis thaliana and its T-DNA insertion mutant ΔAtGLB3 that lacks a functional gene encoding GLB3, a homologue of bacterial truncated Hb
(trHb). Under optimal temperature conditions (e.g. 22°C), the seeds of ΔAtGLB3 and the wild type germinated at a frequency
near 100%. In contrast, at 32°C the seeds of ΔAtGLB3 did not germinate while wild-type seeds retained the same high germination
frequency. The germination of ΔAtGLB3 at 32°C was partially restored by supplementation with the nitric oxide-specific scavenger
2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (carboxy-PTIO; cPTIO), 3-(3,4-dihydroxycinnamoyl)quinic
acid, bovine serum Hb, or isoprene. The results presented in this study suggest that chemical scavengers for reactive nitrogen
species potentially improve seed germination at high temperature. |
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