Characterization of a novel cry2Aa-type gene from Bacillus thuringiensis subsp. kurstaki |
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Authors: | Slim Tounsi Samir Jaoua |
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Affiliation: | (1) Laboratoire des Biopesticides, Centre de Biotechnologie de Sfax, P.O. Box `K', 3038 Sfax, Tunisia |
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Abstract: | A 4 kb BamHI-HindIII fragment, corresponding to the cry2A operon of Bacillus thuringiensis subsp. kurstaki strain BNS3, was cloned. The sequencing of the corresponding cry2Aa-type gene, termed crybns3-4, revealed an open reading frame of 1902 bp, encoding a protein of 633 amino-acid residues. Both nucleotide and amino-acid sequences similarity analysis revealed that crybns3-4 is a new cry2Aa-type gene which has several differences from the reported cry2Aa-type genes. The transfer of the cloned operon to an acrystalliferous mutant of BNS3, revealed an expression of the new cry2Aa-type gene and a production of parasporal crystal inclusions in the transformants. |
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Keywords: | Bacillus thuringiensis cloning cry2Aa DNA sequence expression |
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