Bsr-REMI: An improved method for gene tagging using a new vector inDictyostelium |
| |
Authors: | Takahiro Morio Hiroyuki Adachi Kazuo Sutoh Kaichiro Yanagisawa Yoshimasa Tanaka |
| |
Affiliation: | (1) Institute of Biological Sciences, University of Tsukuba, 305 Tsukuba, Ibaraki, Japan;(2) Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, Meguro-ku, 153 Tokyo, Japan |
| |
Abstract: | Using a plasmid pBsr2 which carries a blasticidin S-resistant gene, we have improved the method of REMI (restriction enzyme-mediated integration) provided for insertional mutagenesis inDictyostelium discoideum (bsr-REMI). To confirm usefulness of thebsr-REMI, transformation efficiency, copy number of integrated DNA, and randomness of integration into genome were examined. |
| |
Keywords: | Bsr-REMI Dictyostelium discoideum Insertional mutagenesis |
本文献已被 SpringerLink 等数据库收录! |
|