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Nitrogen Isotope Fractionation Associated with Nitrate Reductase Activity and Uptake of NO(3) by Pearl Millet
Authors:Mariotti A  Mariotti F  Champigny M L  Amarger N  Moyse A
Affiliation:Université Pierre et Marie Curie, Laboratoire de Géologie dynamique, 75230 PARIS Cedex 05, France.
Abstract:Nitrogen isotope fractionation by Pearl Millet (Pennisetum americanum L. and P. mollissimum L.) grown on nitrate was associated with nitrate reductase activity. Fractionation was evidenced at the step of nitrate reduction when the substrate-to-enzyme ratio was high (possibly saturating for the active sites of the nitrate reductase enzyme), for instance in young seedlings having a low nitrate reductase activity or in seedlings grown on high nitrate concentration.
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