| 野生碧玉兰组培快繁技术 |
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| 引用本文： | 王亚沉,王玉英,周慧恒,李枝林.野生碧玉兰组培快繁技术[J].亚热带植物科学,2013,42(1):27-30. |
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| 作者姓名： | 王亚沉 王玉英 周慧恒 李枝林 |
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| 作者单位： | 云南农业大学园林园艺学院花卉研究所；云南农业大学协同创新中心; |
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| 基金项目： | 国家自然科学基金项目(30760155);科技部科技成果转化项目[国科发农(2012)621];云南省科技攻关项目(2009BB013);云南省重点新产品开发项目(2012BB008) |
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| 摘 要： | 以云南野生碧玉兰（Cymbidium lowianum）种子无菌萌发的试管苗为试验材料，进行其丛生芽诱导增殖、生根的组培快繁技术体系研究。结果表明：适宜的增殖培养基配方为1/2MS＋6-BA 2.0 mg/L＋KT 0.5 mg/L＋NAA 0.2 mg/L，增殖率可达300%；生根培养基的配方为1/2MS＋NAA 1.5 mg/L＋6-BA 0.3 mg/L，其根数多，健壮。
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| 关 键 词： | 碧玉兰 组培 增殖 生根 |
| 收稿时间： | 2013-02-27 |
| 修稿时间： | 2013-02-27 |
Research on Techniques of Tissue Culture and Rapid Propagation of Wild Cymbidium lowianum |
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| WANG Ya-chen,WANG Yu-ying,ZHOU Hui-heng,LI Zhi-lin.Research on Techniques of Tissue Culture and Rapid Propagation of Wild Cymbidium lowianum[J].Subtropical Plant Science,2013,42(1):27-30. |
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| Authors: | WANG Ya-chen WANG Yu-ying ZHOU Hui-heng LI Zhi-lin |
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| Institution: | 1,2(1.Floral Research Institute,Yunnan Agricultural University,Kunming 650201,Yunnan China;2.Collaborative Innovation Center,Yunnan Agricultural University,Kunming 650201,Yunnan China) |
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| Abstract: | With the in vitro germination plantlet of wild Cymbidium lowianum in Yunnan as test material, a series of tissue culture and rapid propagation system research including induced proliferation and rooting for its multiple shoots was carried out. The results showed that the suitable multiplication medium was 1/2MS + 6-BA 2.0 mg/L + KT 0.5 mg/L + NAA 0.2 mg/L, with the proliferation rate of 300%, and the rooting medium was 1/2MS + NAA 1.5 mg/L + 6-BA 0.3 mg/L, then the roots healthy and strong. |
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| Keywords: | Cymbidium lowianum tissue culture proliferation rooting |
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