The effect of cross-linking spectrin-actin complexes with band 4.1 on the state of polymerization of the actin |
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| Authors: | A Husain W H Sawyer G J Howlett |
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| Institution: | Russell Grimwade School of Biochemistry, University of Melbourne, Parkville, Victoria 3052, Australia |
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| Abstract: | The polymerization of actin in the presence of spectrin tetramers and band 4.1 isolated from the human erythrocyte has been measured using a fluorescence energy transfer technique. The results show that the cross-linking of spectrin-actin complexes by band 4.1 results in a limited depolymerization of actin filaments and a concomitant rise in the critical actin concentration. The phenomenon may explain in part the existence of actin in the erythrocyte cytoskeleton as short oligomers rather than as long filaments. |
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| Keywords: | IAEDANS 5-[2-iodacetyl)-aminoethyl]-aminonapthalene-1-sulphonate FM fluorescein-5-maleimide |
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