Staphylolytic enzyme from Pseudomonas aeruginosa: characterization and immunocytochemical localization |
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Authors: | Ana Carnicero Teresa B Mansito José M Roldán Miguel A Falcón |
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Institution: | (1) Departamento de Microbiología y Biología Celular, Facultad de Biología, Universidad de La Laguna, Tenerife, Islas Canarias, Spain;(2) Departamento de Bioquímica, Biología Molecular y Fisiología, Facultad de Ciencias, Universidad de Córdoba, E-14071 Cordoba, Spain |
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Abstract: | Staphylolytic enzyme, a specific peptidase produced by Pseudomonas aeruginosa, has been characterized by using immunochemical procedures. Lytic activity was detected in the extracellular medium of Pseudomonas cultures at the beginning of the stationary growth phase. No activity was detected in bacterial cells. However, lytic protein antigen was present in periplasmic and cytoplasmic fractions, suggesting that staphylolytic enzyme is synthesized as an inactive precursor which becomes active during translocation to the extracellular broth. Results obtained in immunolocalization experiments indicate the presence of the precursor in the outer part of cells. The export pathway of staphylolytic enzyme through the periplasmic space is proposed.Abbreviations DCE
dialyzed crude extract
- CFU
colonies forming units
- LU
lytic unit |
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Keywords: | Staphylolytic enzyme Immunocytochemical localization Pseudomonas aeruginosa Staphylococcus aureus |
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