Automated multi-dimensional purification of tagged proteins |
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Authors: | Sigrell Jill A. Eklund Pär Galin Markus Hedkvist Lotta Liljedahl Pia Markeland Johansson Christine Pless Thomas Torstenson Karin |
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Affiliation: | (1) R&D Separations, Amersham Biosciences AB, Björkgatan 30, 751 84 Uppsala, Sweden |
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Abstract: | The capacity for high throughput purification (HTP) is essential in fields such as structural genomics where large numbers of protein samples are routinely characterized in, for example, studies of structural determination, functionality and drug development. Proteins required for such analysis must be pure and homogenous and available in relatively large amounts. ÄKTAT 3D system is a powerful automated protein purification system, which minimizes preparation, run-time and repetitive manual tasks. It has the capacity to purify up to 6 different His6- or GST-tagged proteins per day and can produce 1–50 mg protein per run at >90% purity. The success of automated protein purification increases with careful experimental planning. Protocol, columns and buffers need to be chosen with the final application area for the purified protein in mind. |
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Keywords: | Ä KTA 3D Kit Ä KTAexplorer HTP, His-tagged proteins automated multi-step protein purification structural genomics |
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