Iberis amara Extract Induces Intracellular Formation of Reactive Oxygen Species and Inhibits Colon Cancer |
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| Authors: | Christopher Weidner Morten Rousseau Annabell Plauth Sylvia J Wowro Cornelius Fischer Heba Abdel-Aziz Sascha Sauer |
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| Institution: | 1Otto Warburg Laboratory, Max Planck Institute for Molecular Genetics, Berlin, Germany;2Scientific Department, Steigerwald Arzneimittelwerk GmbH, Darmstadt, Germany;3CU Systems Medicine, University of Würzburg, Würzburg, Germany;Duke University Medical Center, UNITED STATES |
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| Abstract: | Massively increasing global incidences of colorectal cancer require efficient treatment and prevention strategies. Here, we report unexpected anticancerogenic effects of hydroethanolic Iberis amara extract (IAE), which is known as a widely used phytomedical product for treating gastrointestinal complaints. IAE significantly inhibited the proliferation of HT-29 and T84 colon carcinoma cells with an inhibitory concentration (IC50) of 6 and 9 μg/ml, respectively, and further generated inhibitory effects in PC-3 prostate and MCF7 breast cancer cells. Inhibition of proliferation in HT-29 cells was associated with a G2/M phase cell cycle arrest including reduced expression of various regulatory marker proteins. Notably, in HT-29 cells IAE further induced apoptosis by intracellular formation of reactive oxygen species (ROS). Consistent with predictions derived from our in vitro experiments, bidaily oral gavage of 50 mg/kg of IAE over 4 weeks resulted in significant inhibition of tumor growth in a mouse HT-29 tumor xenograft model. Taken together, Iberis amara extracts could become useful alternatives for preventing and treating the progression of colon cancer. |
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