In vitro synthesis of phosphatidylinositol and phosphatidylcholine by phospholipase D |
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Authors: | Sukhendu B. Mandal Parimal C. Sen Parul Chakrabarti |
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Affiliation: | Department of Chemistry, Bose Institute, Calcutta 700009, India |
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Abstract: | Phosphatidylinositol (PI) was prepared from egg lecithin by a one-step transphosphatidylation reaction catalysed by phospholipase D in the presence of myo-inositol. Similarly phosphatidylcholine (PC) has been synthesized by the same technique from egg phosphatidylethanolamine using phospholipase D and choline chloride.The yield of PI was ca 25 % and that of PC ca 28 %. The transphosphatidylase function of phospholipase D offers a useful route for the synthesis of different classes of phospholipids. |
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Keywords: | Phosphatidylinositol phosphatidylcholine synthesis phospholipase D egg yolk. |
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