Regulation of starch biosynthesis in normal and opaque-2 maize during endosperm development |
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Authors: | Sunita Joshi Madan L. Lodha Shanti L. Mehta |
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Affiliation: | Division of Biochemistry, Nuclear Research Laboratory, Indian Agricultural Research Institute, New Delhi-110012, India |
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Abstract: | The absolute activities of sucrose-UDP glucosyltransferase, glucose-6-phosphate ketoisomerase and soluble and bound ADPG-starch glucosyltransferase have been studied in normal and Opaque-2 maize endosperms during development. In general, the activities of these enzymes except sucrose-UDP glucosyltransferase were higher up to 20 days post-pollination and lower at the 30 day stage in Opaque-2 than in normal maize endosperms. However, sucrose-UDP glucosyltransferase activity was higher in normal maize endosperm up to the 20 day stage while it was lower at subsequent stages than in Opaque-2. It is suggested that the lower level of these enzymes, except sucrose-UDP glucosyltransferase, might be responsible for the reduced accumulation of starch in Opaque-2 endosperm during later stages of endosperm development. |
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Keywords: | Gramineae maize endosperm Opaque-2 sucrose-UDP glucosyltransferase glucose phosphate isomerase starch synthetase. |
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