Glucose-6-phosphate dehydrogenase and 6-phosphogluconic acid dehydrogenase of wild oat seeds |
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Authors: | Stephen W Adkins Peter G Gosling James D Ross |
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Institution: | Department of Botany, University of Reading, Reading, RG6 2AS, Berkshire, U.K. |
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Abstract: | The presence of the initial enzymes of the pentose phosphate pathway, namely glucose-6-phosphate dehydrogenase and 6-phosphogluconic acid dehydrogenase, has been demonstrated in dormant seed of wild oat. Before a partial characterization of these enzymes was made, an inherent NADP-reducing activity and an enzyme deactivating component, both present in the crude extract, were removed by ammonium sulphate precipitation and subsequent desalting. Both enzymes were then shown to be NADP-specific. Typical Michaelis-Menten kinetics were shown by each enzyme towards NADP and their respective substrates. Soluble cytoplasmic dehydrogenase enzymes were present in both embryo and endosperm extracts. |
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Keywords: | Gramineae pentose phosphate metabolism glucose-6-phosphate dehydrogenase 6-phosphogluconic acid dehydrogenase dormancy breakage |
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