Glucosinolates and amines in Reseda media

The content of glucosinolates and amines in green parts of Reseda media has been investigated. Benzylglucosinolate, 2-phenethylglucosinolate, and m-hydroxybenzylglucosinolate occur in appreciable amounts accompanied by minor amounts of other glucosinolates, benzylamine and m-hydroxybenzylamine. Isolation and identification of these compounds was made using ion-exchange chromatography, high voltage electrophoresis, GC, MS, and ¹³C-NMR spectroscopy. The glucosinolates were transformed into corresponding nitriles and isothiocyanates by thioglucoside glucohydrolase-catalysed hydrolysis and to the corresponding carboxylic acids by acid-catalysed hydrolysis. The content of glucosinolates and amines in leaves and inflorescences of R. media has been determined by UV-spectroscopy and GC.