The Time-Course of Hatching Enzyme Secretion in the Sea Urchin Strongylocentrotus purpuratus |
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| Authors: | THOMAS D YAGER DENNIS BARRETT |
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| Institution: | Institute of Molecular Biology, University of Oregon, Eugene, Oregon 97403, USA.;Department of Biological Sciences, University of Denver, Denver, Colorado 80210, USA. |
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| Abstract: | By two independent methods, we have determined approximately the time-course of hatching enzyme secretion in the sea urchin Strongylocentrotus purpuratus . A quick-kill method indicates that a significant fraction of the enzyme is secreted between 90% and 97% of the fertilization-hatching interval. A direct assay method indicates that the remainder of the enzyme is secreted on either side of the 90–97%"window". The entire period of secretion spans from 75% to 100% or more of the fertilization-hatching interval. For embryos raised at 15°C this translates to an interval of 4.8 or more hr. |
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