|
|||||
|
|
Fluorimetric quantification of intracellular lactate dehydrogenase during fermentation using flow injection analysis |
|
| Authors: | M.P. Nandakumar R. Nandakumar B. Mattiasson |
| Affiliation: | (1) Department of Biotechnology, Center for Chemistry & Chemical Engineering, Lund University, P.B. 124, SE- 22100 Lund, Sweden;(2) Present address: Center of Marine Biotechnology, University of Maryland, Baltimore, MD, USA |
| Abstract: | A flow injection system for the on-line detection of the intracellular enzyme lactate dehydrogenase (LDH) during fermentation has been developed. The system is comprised of an on-line cell disintegration part, an immobilised dye based expanded bed column for the affinity capture of LDH and a fluorimetric detection unit. The system with a linearity of 0.1–5.4 U LDH ml–1 was applied for the detection of intracellular accumulation of LDH during Lactococcus lactis subsp.lactis cultivation. |
| Keywords: | flow injection binding assay intracellular enzyme lactate dehydrogenase on-line cell breakage on-line monitoring |
| 本文献已被 SpringerLink 等数据库收录！ | |