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Isolation and characterization of lipids strictly associated to PSII complexes: Focus on cardiolipin structural and functional role |
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| Authors: | A Ventrella G Mascolo A Agostiano |
| Institution: | a Dip. di Chimica, Università di Bari, Via Orabona 4, 70126 Bari, Italy b IPCF-CNR, sez. Bari, Via Orabona 4, 70126 Bari, Italy c IRSA-CNR, sez. Bari, Via F. De Blasio 5, 70123 Bari, Italy d Dip. di Biochim. Medica e Biol. Medica e Fisica Medica, Univ. di Bari, P.zza G. Cesare, 70124 Bari, Italy |
| Abstract: | In this work, lipid extracts from spinach membrane fragments enriched in Photosystem II (PSII) and from spinach PSII dimers were analyzed, by means of Thin Layer Chromatography (TLC) and Electro-Spray Ionization Mass Spectrometry. Cardiolipin found in association with PSII was isolated and purified by preparative TLC, then characterized by mass and mass-mass analyses. Cardiolipin structures with four unsaturated C18 acyl chains and variable saturation degrees were evidenced. Structural and functional effects of different phospholipids on PSII complexes were investigated by Fluorescence, Resonance Light Scattering and Oxygen Evolution Rate measurements. An increment of PSII thermal stability was observed in the presence of cardiolipin and phosphatidylglycerol. |
| Keywords: | CL Cardiolipin Chl Chlorophyll DCBQ 2 5-dichloro-p-benzoquinone DM d-maltoside" target="_blank">n-dodecyl-β-d-maltoside ESI-MS Electro-Spray Ionization Mass Spectrometry ESI-MS-MS Electro-Spray Ionization Mass-Mass Spectrometry LHCI Light Harvesting Complex I LHCII Light Harvesting Complex II MNCB aqueous buffer containing [2-N-morpholine]ethane-sulphonic acid (25 mM) NaCl (10 mM) CaCl2 (5 mM) and NaHCO3 (10 mM) OG d-glucopyranoside" target="_blank">n-octyl-β-d-glucopyranoside OER Oxygen Evolution Rate PA Phosphatidic acid PG Phosphatidylglycerol PI Phosphatidylinositol PSI Photosystem I PSII Photosystem II Qq-TOF Quadrupole quadrupole time of flight RC Reaction Centre RLS Resonance Light Scattering SQDG Sulfoquinovosyldiacylglycerol TLC Thin Layer Chromatography |
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