| 转OsMAPK4基因烟草的抗旱性研究与遗传分析 |
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| 引用本文： | 李杰,齐岩,李莹,刘西燕,冀好布套,朱延明,柏锡,才华. 转OsMAPK4基因烟草的抗旱性研究与遗传分析[J]. 遗传, 2007, 29(9): 1144-1148. DOI: 10.1360/yc-007-1144 |
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| 作者姓名： | 李杰 齐岩 李莹 刘西燕 冀好布套 朱延明 柏锡 才华 |
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| 作者单位： | 东北农业大学生命科学学院, 哈尔滨 150030 |
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| 基金项目： | 国家自然科学基金项目（编号：30471050）资助. |
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| 摘 要： | 以低温处理的水稻辽盐241植株叶片总RNA为模板, 用OsMAPK4基因特异引物通过RT-PCR扩增出OsMAPK4基因, 构建了由E12启动子调控的OsMAPK4基因植物表达载体pBME12。通过农杆菌介导法将OsMAPK4基因导入烟草, 筛选获得25株转基因植株。抗旱性研究结果表明, OsMAPK4基因的超量表达提高了T1代转基因植株的抗旱性。卡那霉素抗性在T1代转基因植株中的分离情况表明, 大多数转基因株系符合单基因遗传规律。
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| 关 键 词： | 遗传 抗旱性 转基因烟草 OsMAPK4基因 |
| 收稿时间： | 2007-03-30 |
| 修稿时间： | 2007-03-302007-06-08 |
Drought resistance and heredity of transgenic tobacco with OsMAPK4 gene |
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| LI Jie,QI Yan,LI Ying,LIU Xi-Yan,JI Haobutao,ZHU Yan-Ming,BAI Xi,CAI Hua. Drought resistance and heredity of transgenic tobacco with OsMAPK4 gene[J]. Hereditas, 2007, 29(9): 1144-1148. DOI: 10.1360/yc-007-1144 |
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| Authors: | LI Jie QI Yan LI Ying LIU Xi-Yan JI Haobutao ZHU Yan-Ming BAI Xi CAI Hua |
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| Affiliation: | Life Science College, Northeast Agricultural University, Harbin 150030, China |
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| Abstract: | OsMAPK4 gene was obtained by RT-PCR using gene special primer from Liaoyan241 leaf treated with low temperature. Plant expression vector pBME12 was constructed in which OsMAPK4 gene was regulated by E12 promoter. OsMAPK4 gene was transferred into tobacco via Agrobacterium-mediated method and 25 transgenic plants obtained. The results of drought resistance experiments showed that overexpression of OsMAPK4 gene conferred enhanced drought resistance of T1 transgenic plants. Kanamycin-resistance segregation in T1 transgenic plants showed that the most transgenic lines were in single gene inheritance. |
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| Keywords: | OsMAPK4 gene tobacco drought resistance heredity |
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