Production of alkaline phosphatase by immobilized growing cells of Escherichia coli excretory mutants |
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| Authors: | Catherine Manin Jean Noël Barbotin Daniel Thomas Jean Claude Lazzaroni |
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| Affiliation: | (1) Laboratoire de Technology EEnzymatique (U.R.A. CNRS 523), Université de Technologie de Compiégne, B.P. 649, F-60206 Compiégne Cedex, France;(2) Atelier de Biotechnologie Enzymatique et Microbienne (U.M. 24), Université Claude Bernard, F-69622 Villeurbanne Cedex, France |
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| Abstract: | Summary In continuous cultures, alkaline phosphatase was synthesised and excreted for more than 250 h by immobilized growing cells in contrast to free cells for which the excretion decreased after 150 h of culture. This observed increase in alkaline phosphatase synthesis and excretion by immobilized cells may have resulted from growing conditions within the gel beads.Offprint requests to: C. Manin |
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