Chemiluminescent analysis of the antioxidant and immunomodulation effects of several psychotropic drugs on peritoneal macrophages. |
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| Authors: | V Hadjimitova T Traykov R Bakalova V Petrova I Lambev H Ohba M Ishikawa Y Baba |
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| Institution: | Department of Medical Physics and Biophysics, Medical University, 2 Zdrave Str., Sofia 1431, Bulgaria. verah@mail.bg |
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| Abstract: | The present study describes the application of several chemiluminescent (CL) methods for evaluation of antioxidant and immunomodulation effects of psychotropic drugs upon phagocytes: KO2-induced luminal-dependent CL for detection of superoxide anion radicals in a pure chemical system; PMA- and A23187-induced CL of peritoneal macrophages for detection of free radicals in cell suspension; and CL, produced by the luciferase-catalyzed luciferin + ATP reaction, for evaluation of cell viability before and after drug application. These methods provide also a way to investigate the location of drug action. It was found that the psychotropic drugs in fluence the 'oxidative burst' of macrophages through two mechanisms: by expression of drug antioxidant properties and/or by a direct immunomodulation effect. |
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| Keywords: | luminal‐dependent chemiluminescence macrophages psychotropic drugs antioxidant activity immunomodulation |
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