The composition of rat liver microsomes. The structural proteins of rat liver microsomes |
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| Authors: | K. A. Ward and J. K. Pollak |
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| Affiliation: | Department of Histology and Embryology, University of Sydney, Sydney, N.S.W. 2006, Australia |
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| Abstract: | 1. The structural-protein component of microsomal membranes was isolated by three separate methods. Analysis by polyacrylamide-gel electrophoresis indicated that the microsomal structural component is made up of a heterogeneous group of proteins. These proteins were further characterized by their phospholipid-binding capacity. The electrophoretic patterns of microsomal structural proteins were found to differ significantly from those of mitochondrial structural proteins. 2. The reticulosomal fraction was also characterized by electrophoresis with reference to total microsomal proteins, microsomal structural proteins and ribosomal proteins. The reticulosomes gave an electrophoretic pattern significantly different from those of the other three preparations examined. It is suggested that reticulosomes consist largely of enzymic proteins of the endoplasmic reticulum. |
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