Expression of p53 gene in B-CLL peripheral lymphocytes. |
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| Authors: | D Gandini S Moretti A Latorraca F Lanza L del Senno |
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| Institution: | Istituto di Chimica Biologica, Centro di Studi Biochimici delle Patologie del Genoma Umano. |
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| Abstract: | The p53 tumor suppressor gene expression has been studied in 23 B-CLL cases at different clinical stages. The analysis failed to show a direct correlation with each stage, but the significantly lower frequency of a BglII RFLP in the pathologic population suggests a role of this gene in B-CLL. Northern Blot analysis showed the expression of p53 mRNA in all the B-CLL cases. A protocol for the RT-PCR methods was set up to study a very small amount of materials which should be better used for sequence analysis. |
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