玉米原料高产γ-聚谷氨酸优良菌株的选育及发酵条件优化

以实验室筛选到的一株枯草芽孢杆菌（Bacillus subtilis）B-1为出发菌株，采用紫外诱变技术对出发菌株进行反复诱变，得到一株能够利用玉米原料生产γ-聚谷氨酸的优良高产菌株B-115，摇瓶发酵γ-聚谷氨酸的产量由原菌株的12.5g/L提高到19.5g/L。再以该菌株为研究对象利用响应面法进行碳源、氮源、谷氨酸钠、金属离子等发酵条件的优化实验，经48h摇瓶发酵，γ-聚谷氨酸产量达到40.98g/ L。

Screening of γ-Polyglutamic Acid High Productive Strain Using Crop Materials and Optimization of Fermentation Condition

One strain Bacillus subtilist B-115 was screened, which can use crop materials to product γ-Polyglutamic acid. The high productive strain B-115 of γ-PGA was obtained from the primitive strain Bacillus subtilis B-1through UV mutagenesis. The yield of γ-Polyglutamic acid increased from original 12.5g/L to 19.5g/L by flask fermentation. In order to enhance the production of this strain, we used Response Surface Methodology (RSM) to optimize fermentation medium ,such as carbon source, nitrogen source, concentration of sodium glutamate, metal ions, the final yield of γ-PGA was upto 40.98g/L.