Culturing satellite cells from living single muscle fiber explants |
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| Authors: | J David Rosenblatt Alison I Lunt David J Parry Terence A Partridge |
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| Institution: | (1) Muscle Cell Biology Group, MRC Clinical Research Building, Royal Postgraduate Medical School, Hammersmith Hospital, Du Cane Road, W12 0NN London, England;(2) Department of Physiology, University of Ottawa, 451 Smyth Road, K1H 8M5 Ottawa, Ontario, Canada |
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| Abstract: | Summary Conventional methods for isolating myogenic (satellite) cells are inadequate when only small quantities of muscle, the tissue
in which satellite cells reside, are available. We have developed a tissue culture system that reliably permits isolation
of intact, living, single muscle fibers with associated satellite cells from predominantly fast and slow muscles of rat and
mouse; maintenance of the isolated fibers in vitro; dissociation, proliferation, and differentiation of satellite cells from
each fiber; and removal of the fiber from culture for analysis. |
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| Keywords: | muscle fiber satellite cell skeletal muscle tissue culture |
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