松栎柱锈菌春孢子多糖体外抗氧化活性

为了研究松栎柱锈菌（Cronartium orientale S.Kaneko）春孢子多糖的体外抗氧化活性，制备了该菌破壁春孢子精制多糖，并对所得春孢子多糖进行了总抗氧化能力、总还原力以及清除二苯代苦味酰基（DPPH·）、清除羟基自由基（·OH）、清除超氧阴离子（O₂^·）的活性进行了测定。体外抗氧化活性分析结果表明：松栎柱锈菌春孢子多糖具有较强的总抗氧化能力；虽然该菌多糖不具有清除多数氧自由基母体（O₂^·）的作用，也不具有清除人工合成自由基DPPH·的作用，但是其能够有效清除对机体破坏力最强的自由基·OH。这表明本文获得的松栎柱锈菌春孢子多糖具有对自由基选择性清除的能力，对其他自由基研究者具有参考价值，可为该菌的开发利用提供参考，也能够为森林有害生物开发利用提供理论依据。

Antioxidant Activities of Polysaccharides from Cronartium orientale Aeciospores in vitro

We prepared the wall-breaking Cronartium orientale aeciospore refined polysaccharides to study the antioxidant activities in vitro, and assayedthe polysaccharides' bioactivities including total antioxidant capacity, total reducing power, and capacities of scavenging 1,1-diphenyl-2-picrylhydrazyl free radicals(DPPH·), scavenging hydroxyl free radical(·OH), scavenging superoxide anion(O₂^·). The polysaccharides' reducing power was lower than Vc, but with a strong total antioxidant capacity.The matrix of most of oxygen free radicals O₂^· and artificial free radical DPPH· could not be scavenged,but it could effectively scavenge the organism's most destructive free radical ·OH. C.orientale aeciospore polysaccharides could scavenge free radicals selectively. Therefore, the polysaccharides possessed potential value on exploitation and could provide reference to other researchers and a theoretical basis for exploitation of forest pests.