灰木莲种源幼苗叶片性状表型多样性分析

以越南收集的12个灰木莲（Manglietia conifera Dandy）种源为材料，对叶片形态性状（叶长、叶宽、叶面积、叶周长、长宽比、叶柄）以及微形态特征（气孔器密度、气孔器长、气孔器宽、气孔器面积、长宽比）进行测定，采用方差分析、变异系数、相关分析和主成分分析等方法进行分析。结果表明：灰木莲不同种源间的叶表型性状存在显著差异；种源中，LC2的叶形态性状的平均变异系数最大（22.09%），TQ2的变异系数最小（12.76%）；叶表型性状中，叶面积的变异系数最大（28.60%），气孔器宽的变异系数最小（5.19%）；相关性分析结果表明叶表型性状间存在显著或极显著的相关关系，而地理因素中经度与叶周长显著相关，经纬度与叶长宽比呈显著相关，海拔与叶表型性状间的相关性不显著（P<0.05）；主成分分析表明前3个主成分的累计贡献率达到了89.29%，基本代表原始性状的全部信息。灰木莲12个种源经聚类分析在欧式距离5阈值处可分为4类。灰木莲种源间的叶表型性状存在着丰富的变异，纬度对灰木莲叶形态特征有明显影响，灰木莲种源间和性状间的变异程度存在着差异，本研究为灰木莲遗传改良提供理论依据。

Phenotypic Diversity Analysis of Seedling Leaf Traits of Manglietia conifera Dandy

It studied the phenotypic variation in the leaves of Manglietia conifera with 12 provenances seedlings from Vietnam, and measured the leaf morphological traits(length, width, area, perimeter, length-width ratio, petiole length) and micro morphological characteristics of stomata(density, length, width, area, length-width ratio) by the variance analysis, variable coefficient(CV), correlation analysis and principal components analysis(PCA). There were significant differences in leaf phenotypic traits among M.conifera provenances. The average CV of leaf morphological traits of provenance LC2 was the maximum(22.09%), and TQ2 was the minimum(12.76%), leaf area recorded the largest CV(28.60%) while stomata width recorded the smallest CV(5.19%). The result of correlation analysis showed that there was significant correlation between leaf phenotypic traits(P<0.05), leaf perimeter was significantly correlated with longitude, and leaf length-width ratio were significantly correlated with longitude and latitude(P<0.05), leaf phenotypic traits were not significantly correlated with longitude and altitude)(P<0.05). By PCA, the cumulative contribution rate of the first 3 principal components was 89.29%, basically represent all the information of the original character. By cluster analysis, the 12 provenances could be clustered into 4 groups at the Euclidean distance of 5. There is abundant variation of leaf phenotypic traits in M.conifera provenances, longitude and latitude have obvious influence on leaf morphological characteristics of M.conifera, and there is difference of variation degree among provenances and leaf phenotypic traits of M.conifera. Our study provides theoretical basis for genetic improvement of M.conifera.