皮诺敛酸/左旋肉碱降低HepG2细胞脂质的最佳浓度配比研究

针对单一活性成分的减肥降脂效果较差，且容易出现耐药性等问题，本文筛选并优化了皮诺敛酸/左旋肉碱降低HepG2细胞脂质的最佳浓度配比，为皮诺敛酸降脂产品的开发提供基础数据。通过油酸诱导HepG2细胞成脂建立体外非酒精性脂肪肝模型。采用油红O染色法确定了皮诺敛酸/左旋肉碱降低HepG2细胞中脂滴的最佳浓度配比。实验结果表明0.5 mmol·L^-1油酸诱导HepG2细胞脂肪变性效果最佳，皮诺敛酸（PLA）最佳降脂浓度为6.25 μmol·L^-1，左旋肉碱（LC）最佳降脂浓度为250 μmol·L^-1，皮诺敛酸/左旋肉碱（PLA/LC）最佳降脂浓度配比为1：40（μmol·L^-1/μmol·L^-1）。皮诺敛酸/左旋肉碱复配物可以显著降低HepG2细胞脂质，具有用量少，协同作用效果突出的优点，因此皮诺敛酸/左旋肉碱复配物在开发降脂减肥产品中具有良好的应用前景。

The Optimized Proportion of Combination Pinoleic Acid/L-Carnitine Decrease Lipid in HepG2

As the single active ingredient is poor and prone to drug resistance on effects of lose weight and lipid-lowering, so the study focuses on screening and optimizing the optimal concentration ratio of pinoleic acid/L-carnitine to reduce the lipid of HepG2 cell, which provides the basic data for the development of pinoleic acid lipid-lowering products. Oleic acid induced HepG2 cell adipogenesis, which was used to simulate the model of nonalcoholic fatty liver in vitro. The optimal concentration in combination of pinoleic acid/L-Carnitine decrease lipid was determinated by oil red O staining. The results show that the optimum concentration of oleic acid-induced HepG2 cell steatosis is 0.5 mmol·L^-1.The optimal lipolysis concentration of pinoleic acid is 6.25 μmol·L^-1. The optimal lipid metabolism concentration of L-Carnitine is 250 μmol·L^-1. The best lipid-lowering ratio of pinoleic acid/L-carnitine(PLA/LC) is 1:40(μmol·L^-1/μmol·L^-1). The characteristics of pinoleic acid and L-Carnitine complexes decreased the doses and improved synergistic effect, Therefore, Combination of pinoleic acid/L-Carnitine has a potential prospectin in the development of lipid-lowering diet products.